Study of Sungkai (Peronema canescens, Jack) Leaf Extract Activity as an Immunostimulators With In vivo and In vitro Methods
نویسندگان
چکیده
Introduction: Sungkai (Peronema canescens, Jack.) contains polysaccharides, terpenoids, alkaloids, and polyphenols which have pharmacological activity as immunostimulants. Objective: This study aimed to see how the effect of extract an immunostimulant agent was carried out in vitro vivo. Materials Methods: conducted using two methods, namely vivo vitro. In research method test phagocytic capacity macrophage cells, percentage leukocytes, total number leukocytes. used 30 male white mice animals that were randomly divided into 5 treatment groups. Each group consisting 6 given different treatments. The negative control with 0.5% NaCMC suspension, substance suspension ethanol various doses 800, 400, 200 mg/kgBW, lastly comparison Stimuno a dose 50 mg/kg orally for 7 days. On day 8, blood taken from mice's vein count its then followed by intraperitoneal injection Staphylococcus aureus bacteria suspension. After 1 hour administration bacterial peritoneal fluid be observed cells. viability immunostimulatory RAW 264.7 cells extraction at concentration 1.10, 100 g/m. cell microtetrazolium (MTT) aims whether sample is safe not toxic observing value should exceed >90%. g/mL found non-toxic Thus, this can performed on LPS induced their levels IL-6 TNF-α. (proinflammatory cytokines) compared alone sandwich ELISA (Enzyme-Linked Immunosorbent Assay) method. Results: observations analyzed one-way ANOVA Duncan's follow-up (significance p<0.05). results showed variations increased significantly (p<0.05) along leukocyte leukocytes had significant increase (p<0.05). It could TNF- tested post hoc test. Conclusion: leaf (Peronemacanescsens of800, mg/kgBW has both
منابع مشابه
a fundamental study of "histiriographic metafiction", and "literary genres", as introduced in "new historical philosophy", and tracing them in the works of julian barnes.
abstract a fundamental study of “historio-graphic metafiction” and “literary genres”, as introduced in “new historical philosophy”, and tracing them in the works of julian barnes having studied the two novels, the porcupine and arthur & george, by julian barnes, the researcher has applied linda hutcheon’s historio-graphic metafictional theories to them. the thesis is divided into five cha...
15 صفحه اولThe antileishmanial activity of Aloe vera leaf exudates: in vitro and in vivo
Background: According to the drug resistance and side effects of the standard treatments for leishmaniasis, achieving effective treatment with less side effects and more benefits is of paramount importance. The present study aimed to evaluate the effect of Aloe vera leaf exudate on Leishmania major under in vitro and in vivo models, in inbred BALB/c mice. Methods: Different concentrations of bo...
متن کاملantioxidant and antimicrobial activity of allium jesdianum extract
چکیده بررسی فعالیت ضد اکسیدانی و ضدمیکروبی عصاره گیاه بن سرخ (allium jesdianum) به کوشش: زهرا مقیمی هدف از این تحقیق بررسی میزان ترکیبات فنولی کل، ترکیبات فلاوونوئیدی کل، فعالیت ضداکسیدانی و ضدمیکروبی عصاره ی گیاه تازه و خشک بن سرخ بود. استخراج عصاره به روش های غوطه وری، استفاده از همزن مغناطیسی، دستگاه مایکروویو، امواج فراصوت و گرمادهی مقاومتی و با استفاده از متانول، اتانول و آب به عنوا...
the study of aaag repeat polymorphism in promoter of errg gene and its association with the risk of breast cancer in isfahan region
چکیده: سرطان پستان دومین عامل مرگ مرتبط با سرطان در خانم ها است. از آنجا که سرطان پستان یک تومور وابسته به هورمون است، می تواند توسط وضعیت هورمون های استروئیدی شامل استروژن و پروژسترون تنظیم شود. استروژن نقش مهمی در توسعه و پیشرفت سرطان پستان ایفا می کند و تاثیر خود را روی بیان ژن های هدف از طریق گیرنده های استروژن اعمال می کند. اما گروه دیگری از گیرنده های هسته ای به نام گیرنده های مرتبط به ا...
15 صفحه اولذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Pharmacognosy Journal
سال: 2021
ISSN: ['0975-3575']
DOI: https://doi.org/10.5530/pj.2021.13.177